Journal: bioRxiv

Article Title: Molecular basis for recognition of the Group A Carbohydrate backbone by the PlyC streptococcal bacteriophage endolysin

doi: 10.1101/2021.03.19.436156

Figure Lengend Snippet: Representation of immunoblot analysis of the cell lysate of E. coli expressing the SCC and GAC pRha and carrying an empty control plasmid (−ve). A) Blot was incubated with PlyCB-Alexa Fluor ® 647 (PlyCB WT AF647 ). B) Probing the same samples with the GAC antibodies confirms the presence of GAC in the bands. Molecular mass markers are given in kDa.

Article Snippet: E. coli genotypes DH5 α (NEB, cat. No. C2988J), DH10 α (Thermo Fisher, cat No. 18297010), BL21 (NEB, cat No. C2530H) and Origami 2 (Millipore Sigma, cat. No. 71346) were used for routine plasmid propagation or protein expression and grown in Lysogeny Broth (LB) medium supplemented with either 50 µg/ml kanamycin, 100 µg/ml ampicillin or 35 µg/ml chloramphenicol as needed.

Techniques: Western Blot, Expressing, Plasmid Preparation, Incubation

Journal: bioRxiv

Article Title: Molecular basis for recognition of the Group A Carbohydrate backbone by the PlyC streptococcal bacteriophage endolysin

doi: 10.1101/2021.03.19.436156

Figure Lengend Snippet: PlyCB binding to E. coli cells were investigated by flow cytometry after labelling with PlyCB WT AF647 and PlyCB R66E AF647 mutant proteins. Blue: −ve control cells without pRha. Red: pRha producing E. coli cells. Representative histograms are shown. A) Left panel: unstained cells. Right panel: The anti-GAC antibodies (GAC-FITC) were used as a positive control to label the E. coli cells producing pRha. The antibodies do not bind to the E. coli cells carrying an empty plasmid (−ve).B) Left panel: PlyCB WT AF647 binds to the E. coli cells producing pRha, but not to the E. coli cells carrying an empty plasmid (−ve). Right panel: PlyCB R66E AF647 does not binds to the E. coli cells producing pRha.

Article Snippet: E. coli genotypes DH5 α (NEB, cat. No. C2988J), DH10 α (Thermo Fisher, cat No. 18297010), BL21 (NEB, cat No. C2530H) and Origami 2 (Millipore Sigma, cat. No. 71346) were used for routine plasmid propagation or protein expression and grown in Lysogeny Broth (LB) medium supplemented with either 50 µg/ml kanamycin, 100 µg/ml ampicillin or 35 µg/ml chloramphenicol as needed.

Techniques: Binding Assay, Flow Cytometry, Mutagenesis, Positive Control, Plasmid Preparation

Journal: Cell reports

Article Title: Loss of FBXO31-mediated degradation of DUSP6 dysregulates ERK and PI3K-AKT signaling and promotes prostate tumorigenesis

doi: 10.1016/j.celrep.2021.109870

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: NEB5- alpha , Promega , Cat. No. C2992H.

Techniques: Virus, Recombinant, Ubiquitin Proteomics, Cell Viability Assay, Viability Assay, Western Blot, CRISPR, Software